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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been observed in several tumors, which ends up in chromosome instability, and so may perhaps lead to tumorigenesis. This protein was observed to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a significant function in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually explained. Two further splice variants ended up claimed but in-depth nucleotide sequence details just isn't nonetheless readily available.
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Blocking peptides are peptides that bind exclusively to the focus on antibody and block antibody binding. These peptide usually is made up of the epitope recognized via the antibody.
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This information outlines even more doable optimization of cellular and immunoassay parameters to make sure the very best success are...
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This guidebook outlines additional feasible optimization of mobile and immunoassay parameters to guarantee the best possible outcomes are...
Antibodies sure to the blocking peptide no more bind towards the epitope on the focus on protein. By comparing the staining within the blocked antibody compared to the antibody alone, one can see which staining is unique.
Goal Facts Cyclin E belongs for the really conserved cyclin household, whose users are characterized by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute towards the temporal coordination of each mitotic occasion. Cyclin E forms a posh with and features being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates at the G1-S period boundary and it is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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